Label-Free and Simultaneous Mechanical and Electrical Characterization of Single Plant Cells Using Microfluidic Impedance Flow Cytometry.
Identifieur interne : 000295 ( Main/Exploration ); précédent : 000294; suivant : 000296Label-Free and Simultaneous Mechanical and Electrical Characterization of Single Plant Cells Using Microfluidic Impedance Flow Cytometry.
Auteurs : Ziyu Han [République populaire de Chine] ; Lincai Chen [République populaire de Chine] ; Shuaihua Zhang [République populaire de Chine] ; Jiehua Wang [République populaire de Chine] ; Xuexin Duan [République populaire de Chine]Source :
- Analytical chemistry [ 1520-6882 ] ; 2020.
Abstract
Despite that single-cell-type-level analyses have been extensively conducted on animal models to gain new insights into complex biological processes; the unique biological and physiological properties of plant cells have not been widely studied at single-cell resolution. In this work, an electrical impedance flow cytometry was fabricated based on microfluidics with constriction microchannel to simultaneously characterize the mechanical and electrical properties of single plant cells. Protoplasts from two model plant species, the herbaceous Arabidopsis thaliana and the woody Populus trichocarpa, could be readily discriminated by their respective mechanical traits, but not by electrical impedance. On the contrary, overexpression of a red fluorescent protein on plasma membrane resulted in changes in cell electrical impedance instead of cell deformability. During primary cell wall (PCW) regeneration, this extracellular layer outside of protoplasts introduced dramatic variations in both mechanical and electrical properties of single plant cells. Furthermore, the effects of auxin, an essential phytohormone regulating PCW reformation, were validated on this platform. Taken together, our results revealed a novel application of microfluidic impedance flow cytometry in the field of plant science to simultaneously characterize dual biophysical properties at single-cell resolution, which could be further developed as a powerful and reliable tool for plant cell phenotyping and cell fate specification.
DOI: 10.1021/acs.analchem.0c02854
PubMed: 32911928
Affiliations:
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Laboratory of Precision Measuring Technology and Instruments, College of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072</wicri:regionArea><placeName><settlement type="city">Tianjin</settlement></placeName></affiliation></author></analytic><series><title level="j">Analytical chemistry</title><idno type="eISSN">1520-6882</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Despite that single-cell-type-level analyses have been extensively conducted on animal models to gain new insights into complex biological processes; the unique biological and physiological properties of plant cells have not been widely studied at single-cell resolution. In this work, an electrical impedance flow cytometry was fabricated based on microfluidics with constriction microchannel to simultaneously characterize the mechanical and electrical properties of single plant cells. Protoplasts from two model plant species, the herbaceous <i>Arabidopsis thaliana</i> and the woody <i>Populus trichocarpa</i>, could be readily discriminated by their respective mechanical traits, but not by electrical impedance. On the contrary, overexpression of a red fluorescent protein on plasma membrane resulted in changes in cell electrical impedance instead of cell deformability. During primary cell wall (PCW) regeneration, this extracellular layer outside of protoplasts introduced dramatic variations in both mechanical and electrical properties of single plant cells. Furthermore, the effects of auxin, an essential phytohormone regulating PCW reformation, were validated on this platform. 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In this work, an electrical impedance flow cytometry was fabricated based on microfluidics with constriction microchannel to simultaneously characterize the mechanical and electrical properties of single plant cells. Protoplasts from two model plant species, the herbaceous <i>Arabidopsis thaliana</i> and the woody <i>Populus trichocarpa</i>, could be readily discriminated by their respective mechanical traits, but not by electrical impedance. On the contrary, overexpression of a red fluorescent protein on plasma membrane resulted in changes in cell electrical impedance instead of cell deformability. During primary cell wall (PCW) regeneration, this extracellular layer outside of protoplasts introduced dramatic variations in both mechanical and electrical properties of single plant cells. Furthermore, the effects of auxin, an essential phytohormone regulating PCW reformation, were validated on this platform. Taken together, our results revealed a novel application of microfluidic impedance flow cytometry in the field of plant science to simultaneously characterize dual biophysical properties at single-cell resolution, which could be further developed as a powerful and reliable tool for plant cell phenotyping and cell fate specification.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Ziyu</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Lincai</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Shuaihua</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jiehua</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-8559-4797</Identifier><AffiliationInfo><Affiliation>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Xuexin</ForeName><Initials>X</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7550-3951</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Chem</MedlineTA><NlmUniqueID>0370536</NlmUniqueID><ISSNLinking>0003-2700</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>11</Day><Hour>5</Hour><Minute>29</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32911928</ArticleId><ArticleId IdType="doi">10.1021/acs.analchem.0c02854</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Tianjin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Han, Ziyu" sort="Han, Ziyu" uniqKey="Han Z" first="Ziyu" last="Han">Ziyu Han</name></noRegion><name sortKey="Chen, Lincai" sort="Chen, Lincai" uniqKey="Chen L" first="Lincai" last="Chen">Lincai Chen</name><name sortKey="Duan, Xuexin" sort="Duan, Xuexin" uniqKey="Duan X" first="Xuexin" last="Duan">Xuexin Duan</name><name sortKey="Wang, Jiehua" sort="Wang, Jiehua" uniqKey="Wang J" first="Jiehua" last="Wang">Jiehua Wang</name><name sortKey="Zhang, Shuaihua" sort="Zhang, Shuaihua" uniqKey="Zhang S" first="Shuaihua" last="Zhang">Shuaihua Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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